Sustainable Bioconversion of Methanol: A Renewable Employing Novel Alcohol Oxidase and Pyruvate Aldolase.

ABSTRACT: Methanol is an ideal one-carbon (C1) feedstock for bioconversion into multicarbon value-added compounds. Biocatalytic approaches to methanol conversion provide sustainable and environmentally friendly alternatives to conventional methods. This process is facilitated by methanol-oxidizing enzymes, including alcohol oxidase (AOx). Here, we report an AOx from Pestalotiopsis fici (PfAOx) with the highest methanol oxidation activity and efficient heterologous expression compared to other AOxs. To investigate the bioconversion of a multicarbon compound (C4 chemical, 2-keto-4-hydroxybutyrate, 2-KHB) from cost-effective methanol, we developed a one-pot enzyme system including PfAOx and pyruvate aldolase from Deinococcus radiodurans (DrADL) with catalase from Bos taurus (BtCAT,

SUBMITTER: Kim JY 

PROVIDER: S-EPMC12007092 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature