SUMOylation of the cardiac sodium channel Na_V1.5 modifies inward current and cardiac excitability.

ABSTRACT:

Background

Decreased peak sodium current (I_Na) and increased late sodium current (I_Na,L), through the cardiac sodium channel Na_V1.5 encoded by SCN5A, cause arrhythmias. Many Na_V1.5 posttranslational modifications have been reported. A recent report concluded that acute hypoxia increases I_Na,L by increasing a small ubiquitin-like modifier (SUMOylation) at K442-Na_V1.5.

Objective

The purpose of this study was to determine whether and by what mechanisms SUMOylation alters I_Na, I_Na,L, and cardiac electrophysiology.

Methods

SUMOylation of Na_V1.5 was detected by immunoprecipitation and immunoblotting. I_Na was measured by patch clamp with/without SUMO1 overexpression

SUBMITTER: Yoon JY 

PROVIDER: S-EPMC12007604 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature