Antigen Presentation by Extracellular Vesicles in the Acute Phase of Corneal Transplantation.

ABSTRACT:

Purpose

Corneal transplantation is the most frequently performed solid organ transplantation procedure worldwide. We aimed to investigate the role of extracellular vesicles in allorecognition and the rejection of allografts in corneal transplantation using male C57BL/6 (B6 and IAb) and BALB/c (IAd) mice.

Methods

The mice were aged 8 to 12 weeks. We used transmission electron microscopy to visualize the drainage of extracellular vesicles derived from the graft into the cervical lymph nodes using gold colloids as markers in a mouse model of corneal transplantation.

Results

Lymph node cells sensitized to graft-derived extracellular vesicles exhibited high proliferation when exposed to cultured corneal stromal cells in a mixed lymphocyte reaction. Notably, extracellular v

SUBMITTER: Shimizu T 

PROVIDER: S-EPMC12007676 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature