¹³C-metabolic flux analysis of <i>Saccharomyces cerevisiae</i> in complex media.

ABSTRACT: Saccharomyces cerevisiae is often cultivated in complex media for applications in food and other biochemical production. However, ¹³C-metabolic flux analysis (¹³C-MFA) has been conducted for S. cerevisiae cultivated in synthetic media, resulting in a limited understanding of the metabolic flux distributions under the complex media. In this study, ¹³C-MFA was applied to S. cerevisiae cultivated in complex media to quantify the metabolic fluxes in the central metabolic network. S. cerevisiae was cultivated in a synthetic dextrose (SD) medium supplemented with 20 amino acids (SD + AA) and yeast extract peptone dextrose (YPD) medium. The results revealed that glutamic acid, glutamine, aspartic acid, and asparagine are incorporated in

SUBMITTER: Fujiwara H 

PROVIDER: S-EPMC12008597 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature