New Approaches Based on Serial-Block Face Electron Microscopy to Investigate the Peripheral Nervous System.

ABSTRACT:

Background and aims

Serial block face scanning electron microscopy (SBF-SEM) enables automated 3D imaging of specimens with ultrastructural resolution. However, its application is often restricted due to the complex and labor-intensive nature of the processes involved. This study addresses the challenges associated with sample preparation and the final 3D reconstruction for ultrastructural analysis of peripheral nerves and dorsal root ganglia (DRG) specimens.

Methods

Specimens from the caudal nerve and DRG of mice were prepared for SBF-SEM using three different techniques: (1) manual high molecular weight staining, regarded as the gold standard, (2) automated standard transmission electron microscopy (TEM) preparation, and (3) automated uranyl-free en bloc preparation. The a

SUBMITTER: Borisovs V 

PROVIDER: S-EPMC12008736 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature