Tripartite motif-containing 27 negatively regulates NF-κB activation in bone remodeling.

ABSTRACT:

Background

Tripartite motif-containing 27 (TRIM27) is highly expressed in the mouse thymus, spleen, and hematopoietic compartment cells and regulates cell proliferation, apoptosis, and innate immune responses. However, the role of TRIM27 in bone remodeling remains unknown. This study aimed to investigate the role of TRIM27 in the differentiation of osteoclasts and osteoblasts.

Methods

We measured the effects of overexpression or knockdown of TRIM27 in osteoclasts and osteoblasts using real-time PCR and Western blot analysis to quantify the mRNA and protein levels of marker genes. Additionally, we performed an in vivo analysis of TRIM27 knockout mice through bone mineral density analysis and histological analysis.

Results

TRIM27 deficiency decreased bone mineral densit

SUBMITTER: Kim K 

PROVIDER: S-EPMC12008848 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature