Inhibition of ceramide synthesis improves the outcome of ischemia/reperfusion injury in cardiomyocytes derived from human induced pluripotent stem cell.

ABSTRACT:

Background

Ceramides are bioactive sphingolipids that have physiological effects on inflammation, apoptosis, and mitochondrial dysfunction. They may play a critical role in the harm of ischemia/reperfusion (IR). Ceramides and IR injury are not well-studied, and there is a lack of human data.

Methods and results

Current studies aimed to investigate the role of ceramide buildup in cardiomyocytes (CMs) death using CMs derived from human induced pluripotent stem cell (hiPSC) as a model for simulating IR injury in vitro. In our model, serum- and glucose-free media was used to expose hiPSC-derived CMs to hypoxia (3% O₂) for 6 h (hrs), followed by reoxygenation (20% O₂) for 16 h. In contrast to normoxia (control) or hypoxia (ischemia), our data showed that fol

SUBMITTER: Haxhikadrija P 

PROVIDER: S-EPMC12008854 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature