MLKL is involved in the regulation of skin wound healing and interplay between macrophages and myofibroblasts in mice.

ABSTRACT: Mixed lineage kinase domain-like protein (MLKL), a critical necroptosis effector, is strongly linked to inflammation, a key component of skin wound healing. However, its precise role in the wound healing process remains inadequately characterized. This study revealed sustained MLKL overexpression throughout the wound healing process, not limited to the early inflammation phase. Wound healing was delayed in MLKL-deficient (MLKL^-/-) mice compared to wild type C57BL/6J (MLKL^+/+) mice, with impaired morphological and pathological recovery. MLKL deficiency reduced the synthesis of inflammatory factors (IL-6, TNF-α, PGE₂), tissue repair molecules (EGF, VEGF, ERα, MMP-9), and apoptosis markers (Caspase-3, p53, Bcl-2) at wound site. Subsequently, a co-culture syste

SUBMITTER: Zhao J 

PROVIDER: S-EPMC12009362 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature