Localizatome: a database for stress-dependent subcellular localization changes in proteins.

ABSTRACT: Understanding protein subcellular localization and its dynamic changes is crucial for elucidating cellular function and disease mechanisms, particularly under stress conditions, where protein localization changes can modulate cellular responses. Currently available databases provide insights into protein localization under steady-state conditions; however, stress-related dynamic localization changes remain poorly understood. Here, we present the Localizatome, a comprehensive database that captures stress-induced protein localization dynamics in living cells. Using an original high-throughput microscopy system and machine learning algorithms, we analysed the localization patterns of 10 287 fluorescent protein-fused human proteins in HeLa cells before and after exposure to oxidative stress.

SUBMITTER: Matsushima T 

PROVIDER: S-EPMC12010962 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature