Modal focal adaptive optics for Bessel-focus two-photon fluorescence microscopy.

ABSTRACT: Adaptive optics (AO) improves the spatial resolution of microscopy by correcting optical aberrations. While its application has been well established in microscopy modalities utilizing a circular pupil, its adaptation to systems with non-circular pupils, such as Bessel-focus two-photon fluorescence microscopy (2PFM) with an annular pupil, remains relatively uncharted. Herein, we present a modal focal AO (MFAO) method for Bessel-focus 2PFM. Measuring and correcting aberration using a spatial light modulator placed in conjugation with the focal plane of the microscope objective, MFAO employs Zernike annular polynomials - a first in AO implementation - to achieve performance on par with a previous zonal AO method, but with a notably simplified optical configuration. We validated the performan

SUBMITTER: Kim H 

PROVIDER: S-EPMC12011382 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature