Comparative urine proteomic study involving papillary thyroid carcinoma and benign thyroid nodules.

ABSTRACT:

Introduction

Accurately differentiating benign and malignant lesions is essential for treatment. We aimed to determine differences in urine proteomics between papillary thyroid carcinomas (PTCs) and benign thyroid nodules (BTNs) and identify biomarkers for the differential diagnosis of these diseases.

Methods

We collected 155 specimens. In the discovery group, 30 PTC and 31 BTN specimens were quantitatively compared using liquid chromatography-tandem mass spectrometry (MS). The diagnostic value of each significantly altered protein was calculated in the MS validation comprising 11 PTC and 10 BTN samples. Ultimately, 36 BTN and 37 PTC specimens were used for ELISA validation.

Results and discussion

Overall, 2,479 proteins were used for quantitative analysis. Compared w

SUBMITTER: Wei L 

PROVIDER: S-EPMC12011787 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature