Single-cell transcriptomic analysis identified resistant MDSCs and a stress-tolerant gene co-expression network as common MDSC features across multiple disease settings.

ABSTRACT:

Background

Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population of immunosuppressive myeloid cells. The identification of a molecular signature common to MDSC regardless of tissue source would aid in the classification of cells as MDSCs.

Methods

Single-cell RNA sequencing (scRNA-seq) was performed on GM-CSF+ IL-6-induced human MDSCs to characterize the extent of heterogeneity within monocytic MDSCs (M-MDSCs). Cytokine-treated PBMCs were also cultured in the absence of serum to include an additional element of cell stress. Independent published bulk and single-cell transcriptomic datasets were used for validation.

Findings

A cluster of cells with preserved MDSC features was induced by the combination of inflammatory signals and cell stress in the for

SUBMITTER: Chen T 

PROVIDER: S-EPMC12011849 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature