Mass spectrometry-based characterisation of the cardiac microtissue metabolome and lipidome.

ABSTRACT:

Introduction

The use of large, non-sample specific metabolite reference libraries often results in high proportions of false positive annotations in untargeted metabolomics.

Objective

This study aimed to measure and curate a library of polar metabolites and lipids present in cardiac microtissues.

Results

Untargeted ultra-high performance liquid chromatography-coupled mass spectrometry measurements of cardiac microtissue intracellular extracts were annotated by comparison against four spectral databases and a retention time library. The annotations were combined to create a library of 313 polar metabolites and 1004 lipids.

Conclusions

The curated library will facilitate higher confidence metabolite annotation in mass spectrometry-based untargeted metabolomics of

SUBMITTER: Bowen TJ 

PROVIDER: S-EPMC12011886 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature