Metabolomic and functional analyses of small molecules secreted by intestinal nematodes in the activation of epithelial tuft cells.

ABSTRACT:

Introduction

Intestinal helminth parasites trigger the host immune response through epithelial sensory tuft cells, but helminth-derived molecules that may activate tuft cells are poorly characterized.

Objectives

The study aimed to identify small molecules released in vitro by two nematode parasites, that infect rodents (Nippostrongylus brasiliensis) and ruminants (Haemonchus contortus), and to test candidate ligands in an in vivo model of tuft cell differentiation.

Methods

Small molecules were analyzed by hydrophilic interaction liquid chromatography (HILIC) of material released by adult parasites incubated in serum-free media, followed by mass spectrometry; selected molecules were administered to mice and tuft cell expansion enumerated after 5 days.

Results

A

SUBMITTER: Poveda MC 

PROVIDER: S-EPMC12011944 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature