Creation of a highly stable direct electron transfer-type enzyme sensor by combining a hyperthermophilic dehydrogenase and natural electron mediator.

ABSTRACT: This study aimed to address the stability limitations of third-generation biosensors using enzymes from mesophilic organisms, by engineering a stable direct electron transfer (DET)-type dehydrogenase capable of transferring electrons extracted from the substrate to the electrode. A fusion protein combining the mediated electron transfer (MET)-type aldose sugar dehydrogenase from the hyperthermophile Pyrobaculum aerophilum (PaeASD), which cannot transfer electrons generated by enzymatic reactions to the electrode without a mediator, and the natural electron transfer protein cytochrome b₅₆₂ (cyt b₅₆₂) was developed to investigate its potential for the DET reaction. A recombinant protein expression system was established in Escherichia coli to produce the PaeASD-cyt b

SUBMITTER: Maeno M 

PROVIDER: S-EPMC12011955 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature