LncRNA NEAT1 sponges miR-214-3p to promote osteoblast differentiation through regulating the PI3K/AKT/mTOR pathway in aortic valve calcification.

ABSTRACT: Calcific aortic valve disease (CAVD) is the common valvular disease associated with significant morbidity and mortality. Dysregulation of long non-coding RNA (lncRNA) has been implicated in the pathogenesis of CAVD. This study aims to investigate the role of NEAT1 in CAVD pathogenesis. NEAT1, miR-214-3p and mRNA expressions were determined by qRT-PCR. Protein expressions were detected by Western blotting. Mineralized bone matrix formation was assessed by Alizarin Red staining. The osteogenic phenotype was evaluated by the alkaline phosphatase activity assay. Dual-luciferase assays were employed to confirm the binding interactions between NEAT1 and miR-214-3p, miR-214-3p and PTEN. NEAT1 was up-regulated in calcific aortic valves and after osteogenic induction of valve interstitial cells (VI

SUBMITTER: Xu R 

PROVIDER: S-EPMC12012154 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature