Proximity-activated DNA scanning encoded sequencing for massive access to membrane proteins nanoscale organization.

ABSTRACT: Cellular structure maintenance and function regulation critically depend on the composition and spatial distribution of numerous membrane proteins. However, current methods face limitations in spatial coverage and data scalability, hindering the comprehensive analysis of protein interactions in complex cellular nanoenvironment. Herein, we introduce proximity-activated DNA scanning encoded sequencing (PADSE-seq), an innovative technique that utilizes flexible DNA probes with adjustable lengths. These dynamic probes are anchored at a single end, enabling free swings within a nanoscale range to perform global scanning, recording, and accumulating of information on diverse proximal proteins in random directions along unrestricted paths. PADSE-seq leverages th

SUBMITTER: Zhao X 

PROVIDER: S-EPMC12012555 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature