Benchmark Dose Estimation from Transcriptomics Data for Methylimidazolium Ionic Liquid Hepatotoxicity: Implications for Health Risk Assessment of Green Solvents.

ABSTRACT: Ionic liquids (ILs), traditionally considered environmentally benign solvents, have shown potential toxicity to organisms, raising concerns about their safety. Among them, 1-octyl-3-methylimidazolium (M8OI) has been detected at high concentrations in soils and exhibits hepatotoxic properties. To uncover the molecular mechanisms underlying this toxicity, whole-transcriptome sequencing was performed, coupled with benchmark dose (BMD) modeling, to derive transcriptomic points-of-departure (tPOD) through dose-response analysis. The transcriptomic analysis identified 425, 667, and 567 differentially expressed genes (DEGs) following low (10 μmol/L), medium (50 μmol/L), and high (200 μmol/L) doses of M8OI exposure, respectively. Enrichment analysis revealed significant perturbations in pathways r

SUBMITTER: Yang Q 

PROVIDER: S-EPMC12012654 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature