Orchestration of human multi-lineage hematopoietic cell development by humanized in vivo bone marrow models.

ABSTRACT: Hematopoiesis develops in the bone marrow (BM) where multiple interactions regulate the differentiation and preservation of hematopoietic stem and progenitor cells (HSPCs). Immune-deficient murine models have enabled the analysis of molecular and cellular regulation of human HSPCs, but the physiology of these models is questioned as human hematopoietic cells develop in xenogenic microenvironments. In this study, we thoroughly characterized a humanized (h) in vivo BM model, developed from fetal (F/) and post-natal (P-N/) mesenchymal stromal cell (MSC) differentiation (called hOssicles [hOss]), in which human hematopoietic cells are generated following the transplantation of CD34⁺ cells. Serial isolation and transplant experiments of hMSCs and HSPCs from hOss revealed the dynamic

SUBMITTER: Renou L 

PROVIDER: S-EPMC12012840 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature