FOXO1 mediates miR-99a-5p/E2F7 to restrain breast cancer cell proliferation and induce apoptosis.

ABSTRACT:

Background

FOXO1 is known to act as a tumor suppressor gene in breast cancer, but its exact mechanism of action remains unclear.

Objective

This study aimed to clarify how FOXO1 suppresses breast cancer cell proliferation and induces apoptosis.

Methods

Breast cancer cell lines were generated with stable knockdown or overexpression of FOXO1. RT-qPCR and western blot assays were conducted to confirm transfection efficiency. CCK-8 and colony formation assays were used to assess cell proliferation, while flow cytometry measured apoptosis. The cells were subcutaneously injected into nude mice, and the volume and mass of the resulting tumors were evaluated. Immunohistochemistry was used to analyze Ki-67 expression in the tumors. A TUNEL assay examined apoptosis in the tumor

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12013025 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature