In vitro RNA-mediated gene silencing of Fusarium oxysporum f.sp. cubense from Ecuador and assessment of RNAi molecule stability in banana plants.

ABSTRACT:

Objective

Fusarium wilt poses a significant threat to banana production, caused by diverse clonal Fusarium lineages. Given the lack of curative measures, developing effective treatments is crucial. RNA interference (RNAi) technology, utilizing double-stranded RNA (dsRNA) molecules, offers a promising solution. In this study, RNAi was evaluated by silencing the activity of the Beta-tubulin (Focβ-tub), C5 Sterol desaturase (FocERG3) and Chitin synthase 1 (FocChs1) genes in a pathogenic Fusarium strain. Furthermore, we study the potential of dsRNA translocation in 3 months old banana plants at early hours of been spray under greenhouse conditions.

Results

In vitro results demonstrated that dsRNA-FocChs1 was more effective in inhibiting spores, with an average IC₅₀ of

SUBMITTER: Pacheco R 

PROVIDER: S-EPMC12013083 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature