Interactions between the archaeal Cdc6 and MCM proteins modulate their biochemical properties.

ABSTRACT: The origin recognition complex, Cdc6 and the minichromosome maintenance (MCM) complex play essential roles in the initiation of eukaryotic DNA replication. Homologs of these proteins may play similar roles in archaeal replication initiation. While the interactions among the eukaryotic initiation proteins are well documented, the protein-protein interactions between the archaeal proteins have not yet been determined. Here, an extensive structural and functional analysis of the interactions between the Methanothermobacter thermautotrophicus MCM and the two Cdc6 proteins (Cdc6-1 and -2) identified in the organism is described. The main contact between Cdc6 and MCM occurs via the N-terminal portion of the MCM protein. It was found that Cdc6-MCM interaction, but not Cdc6-DNA binding, plays the

SUBMITTER: Kasiviswanathan R 

PROVIDER: S-EPMC1201339 | biostudies-literature | 2005

REPOSITORIES: biostudies-literature