Multimodal imaging of a liver-on-a-chip model using labelled and label-free optical microscopy techniques.

ABSTRACT: A liver-on-a-chip model is an advanced complex in vitro model (CIVM) that incorporates different cell types and extracellular matrix to mimic the microenvironment of the human liver in a laboratory setting. Given the heterogenous and complex nature of liver-on-a-chip models, brightfield and fluorescence-based imaging techniques are widely utilized for assessing the changes occurring in these models with different treatment and environmental conditions. However, the utilization of optical microscopy techniques for structural and functional evaluation of the liver CIVMs have been limited by the reduced light penetration depth and lack of 3D information obtained using these imaging techniques. In this study, the potential of both labelled as well as label-free multimodal optical imagin

SUBMITTER: Majer J 

PROVIDER: S-EPMC12013482 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature