Programmable hyperspectral coherent anti-Stokes Raman scattering microscopy.

ABSTRACT: Hyperspectral coherent Raman scattering microscopy provides a significant improvement in acquisition time compared to spontaneous Raman scattering yet still suffers from the time required to sweep through individual wavenumbers. To address this, we present the use of a pulse shaper with a 2D spatial light modulator for phase- and amplitude-based shaping of the Stokes beam to create programmable spectrally tailored excitation envelopes. This enables collection of useful spectral information in a more rapid and efficient manner.

SUBMITTER: Sorrells JE 

PROVIDER: S-EPMC12013496 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature