RNA-Sequencing Reveals the Modulation of the NLRP3 Inflammasome by miR-223-3p in Extracellular Vesicles in Bacterial Endophthalmitis.

ABSTRACT:

Purpose

Extracellular vesicles (EVs) are critical mediators of cell-cell communication via transfer of molecular cargo including miRNAs and regulate transcription in various physiological and pathological conditions. This study aimed to investigate the role of EV-derived-microRNAs (EV-miRNAs) in bacterial endophthalmitis, focusing on their regulatory impact on inflammation and host immune responses.

Methods

C57BL/6 mice were infected with Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) to induce endophthalmitis. EVs were isolated and characterized followed by miRNA profiling to identify differentially expressed miRNAs. The miRNet platform was used to elucidate potential interactions between exosomal miRNA and retinal mRNA, followed by in vivo and

SUBMITTER: Rudraprasad D 

PROVIDER: S-EPMC12013669 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature