Application of Metagenomic Long-Read Sequencing for the Diagnosis of Herpetic Uveitis.

ABSTRACT:

Purpose

To investigate the sensitivity and specificity of herpes virus detection by nanopore metagenomic analysis (NMA) compared with multiplex polymerase chain reaction (mPCR)-positive and -negative controls.

Methods

This study included 43 patients with uveitis who had been screened for intraocular herpes virus infection using mPCR from aqueous humor samples. Aqueous humor samples stored after mPCR were subjected to whole-genome amplification, long-read sequencing, and analysis of the phylogenetic microorganism composition using a Flongle flow cell on the Oxford Nanopore MinION platform. For samples that tested positive with mPCR and negative with the Flongle flow cell, additional long-read sequencing was performed using a MinION flow cell, which enabled acquisition of more

SUBMITTER: Koyanagi Y 

PROVIDER: S-EPMC12013679 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature