Assessing anti-rabies vaccine response in humans: A rapid and high-throughput adaptable, pseudovirus-based neutralization assay as an alternative to rapid fluorescent focus inhibition test (RFFIT).

ABSTRACT:

Background

Rabies is a fatal, but vaccine-preventable disease. The conventional 'gold standard' recommended for estimating anti-rabies vaccine immune response is Rapid Fluorescent Focus Inhibition Test (RFFIT). Here, we describe a pseudovirus-based neutralization assay (PVNT) as an alternative method to detect the neutralizing antibody response.

Methodology/principal findings

We used a recombinant Vesicular Stomatitis Virus (rVSV)-based pseudotyping system in HEK293 cells transfected with rabies G glycoprotein to produce high titre pseudoviruses. These were confirmed by neutralization with anti-G monoclonal antibodies and a reference serum, as well as immunofluorescence-based detection of pseudovirus infection. A secreted alkaline phosphatase (SEAP) reporter was included to

SUBMITTER: Lupitha SS 

PROVIDER: S-EPMC12013880 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature