Proteomics analysis of H-RAS-mediated oncogenic transformation in a genetically defined human ovarian cancer model.

ABSTRACT: RAS is a small GTP binding protein mutated in approximately 30% human cancer. Despite its important role in the initiation and progression of human cancer, the underlying mechanism of RAS-induced human epithelial transformation remains elusive. In this study, we probe the cellular and molecular mechanisms of RAS-mediated transformation, by profiling two human ovarian epithelial cell lines. One cell line was immortalized with SV40 T/t antigens and the human catalytic subunit of telomerase (T29), while the second cell line was transformed with an additional oncogenic ras(V12) allele (T29H). In total, 32 proteins associated with RAS-mediated transformation have been identified using peptide mass fingerprinting. These protein targets are involved in several cellular pathways, including metabol

SUBMITTER: Young T 

PROVIDER: S-EPMC1201440 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature