Combined transcriptomics and metabolomics analyses reveal the molecular mechanism of heat tolerance in <i>Pichia kudriavzevii</i>.

ABSTRACT:

Introduction

Pichia kudriavzevii is a prevalent non-Saccharomyces cerevisiae yeast in baijiu brewing. The aim of this study was to isolate a high temperature resistant Pichia kudriavzevii strain from the daqu of strong flavor baijiu and to elucidate its molecular mechanism.

Methods

Growth activity was assessed at temperatures of 37°C, 40°C, 45°C, and 50°C. Morphological changes were observed by scanning electron microscopy at 37°C, 45°C, and 50°C. Subsequent analysis of the transcriptomics and metabolomics was undertaken to elucidate the molecular mechanism of heat tolerance.

Results

The strain was able to tolerate high temperature of 50°C, undergoing substantial morphological alterations. Gene ontology (GO) analysis of the tr

SUBMITTER: Wang N 

PROVIDER: S-EPMC12014439 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature