Proteomic analysis of infiltrating neutrophils from rheumatoid arthritis synovial fluid and their contribution to protein carbamylation.

ABSTRACT:

Introduction

Carbamylated proteins and dysregulated neutrophils are implicated in rheumatoid arthritis (RA) pathogenesis. Herein, we characterized the neutrophils present in RA synovial fluid (SF) using proteomic techniques and evaluated their contribution to protein carbamylation.

Methods

RA-SF neutrophil proteomic profile and SF proteome signature were investigated using high-resolution mass spectrometry. Carbamylated proteins and the degree of protein carbamylation were evaluated by mass spectrometric analysis. ELISA and chemiluminescence kits were used to examine myeloperoxidase (MPO) activity, and hydrogen peroxide (H₂O₂) generation.

Results

SF neutrophils exhibited a shift in proteomic cargo with up-regulated proteins involved in defense re

SUBMITTER: Chen X 

PROVIDER: S-EPMC12014540 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature