Serodiagnosis of equine infectious anemia by indirect ELISA based on a novel synthetic peptide derived from gp45 glycoprotein.

ABSTRACT: The transmembrane glycoprotein gp45 (implicated in virus-equine B lymphocyte binding and in subsequent infection) is an important protein used as an antigen in the serological diagnosis of equine infectious anemia, but synthetic peptides derived from its structure are not. Consequently, the present study aimed to determine the diagnostic performance of the Bio-AIELAB immunoassay (novel synthetic peptide P05/gp45) in comparison with a similar commercial assay (ELISA EIA, VMRD Inc, USA) and with the gold standard (AGID, LABIOFAM, Cuba). A total of 365 samples of sera from naturally infected Cuban horses (positive: 179, and negative: 186) were investigated by Bio-AIELAB and AGID, of which 299 (positive: 147, and negative: 152) were analysed by ELISA EIA. The performance indicators used in all

SUBMITTER: Dominguez-Odio A 

PROVIDER: S-EPMC12014826 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature