Biocatalytic production of 3-hydroxypropionic acid precursors using a regioselective Baeyer-Villiger monooxygenase.

ABSTRACT: Baeyer-Villiger monooxygenases (BVMOs) are versatile biocatalysts that catalyse the oxidation of ketones to esters with high regio- and enantioselectivity, operating under mild reaction conditions while reducing hazardous waste. Some BVMOs can convert cellulose-derived alkyl levulinates to 3-acetoxypropionates (3-APs), which are key intermediates in the production of 3-hydroxypropionic acid (3-HP), a versatile building block chemical. In this study, a BVMO from Acinetobacter radioresistens (Ar-BVMO) was tested as a biocatalyst for the conversion of three marketed alkyl levulinates: methyl, ethyl and butyl levulinate. The enzyme showed 4-fold higher catalytic efficiency (k_cat/K_M) and enhanced regioselectivity for the desired 3-AP product (4:1 ratio) when using butyl le

SUBMITTER: De Angelis M 

PROVIDER: S-EPMC12015234 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature