Engineering coupled consortia-based biosensors for diagnostic.

ABSTRACT: Synthetic multicellular systems have great potential for performing complex tasks, including multi-signal detection and computation through cell-to-cell communication. However, engineering these systems is challenging, requiring precise control over the cell concentrations of distinct members and coordination of their activity. Here, we develop a bacterial consortia-based biosensor for Heme and Lactate, wherein members are coupled through a global shared quorum-sensing signal that simultaneously controls the activity of the diverse biosensing strains. The multicellular system incorporates a gene circuit that computes the minimum between each biosensor's activity and the shared signal. We evaluate three consortia configurations: one where the shared signal is externally supplied, another di

SUBMITTER: Huang R 

PROVIDER: S-EPMC12015303 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature