Lipoprotein Lpp and L, D-transpeptidases regulate the master regulator of virulence AggR in EAEC.

ABSTRACT: Enteroaggregative Escherichia coli (EAEC) is a diarrheagenic pathotype associated with traveler's diarrhea, foodborne outbreaks, and sporadic diarrhea in industrialized and developing countries. Regulation of virulence factors in EAEC is mediated by the master regulator AggR, an AraC/XylS family member controlling the expression of more than 44 genes associated with metabolism and virulence. Although the AggR regulon is well-characterized, the mechanism and upstream signaling cascades that regulate its activation are poorly understood. This study demonstrates that Lpp (Braun's lipoprotein) and L, D-transpeptidases are required for AggR activation. We found that deletion lpp in EAEC resulted in the downregulation of more than 100 genes involved in transport, metabolism, and virulence. Among

SUBMITTER: Rodriguez-Valverde D 

PROVIDER: S-EPMC12015436 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature