A chromosome-scale and haplotype-resolved genome assembly of tetraploid blackberry (<i>Rubus</i> L. subgenus <i>Rubus</i> Watson).

ABSTRACT: Blackberries (Rubus spp.) are globally consumed and well known for their rich anthocyanin and antioxidant content and distinct flavors. Improving blackberries has been challenging due to genetic complexity of traits and limited genomic resources. The blackberry genome has been particularly challenging to assemble due to its polyploid nature. Here, we present the first chromosome-scale and haplotype-phased assembly for the primocane-fruiting, thornless tetraploid blackberry selection BL1 (Rubus L. subgenus Rubus Watson). The genome assembly was generated using Oxford Nanopore Technology and Hi-C scaffolding, resulting in a 919 Mb genome distributed across 27 pseudochromosomes, with an N50 of 35.73 Mb. This assembly covers >92% of the genome length and contains over 98%

SUBMITTER: Paudel D 

PROVIDER: S-EPMC12015472 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature