A novel approach in using insect-based spinach-food waste for gene targeting to cancer tissues.

ABSTRACT: In our study, we prepared Fe₃O₄ nanoparticles (NPs) using food waste extract of Mealworm (Tenebrio molitor) larvae fed spinach (Spinacia oleracea), which is rich in iron. A coating was applied to Fe₃O₄ NPs containing hyperbranched spermine-polyethylene glycol-folic acid (FHSPF) and spermine-polyethylene glycol-folic acid (FSMPF). Polymer was loaded with siRNA or DNA. DLS¹, H-NMR, FTIR, EDX, Zeta potential and TEM were used to analyze morphology of NPs. Biocompatibility, DNA release, and gene transfer properties were evaluated. Coats concentration in our NPs increased zeta potential, DNA release, encapsulation, and gene delivery efficiency. As determined by cell viability, our NPs exhibit low cytotoxicity and good compatibility; on the

SUBMITTER: Majd-Marani S 

PROVIDER: S-EPMC12015529 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature