Regulation of mouse hepatic genes in response to diet induced obesity, insulin resistance and fasting induced weight reduction.

ABSTRACT:

Background

Obesity is associated with insulin resistance that can often be improved by caloric restriction and weight reduction. Although many physiological changes accompanying insulin resistance and its treatment have been characterized, the genetic mechanisms linking obesity to insulin resistance are largely unknown. We used DNA microarrays and RT-PCR to investigate significant changes in hepatic gene transcription in insulin resistant, diet-induced obese (DIO)-C57/BL/6J mice and DIO-C57/BL/6J mice fasted for 48 hours, whose weights returned to baseline levels during these conditions.

Results

Transcriptional profiling of hepatic mRNA revealed over 1900 genes that were significantly perturbed between control, DIO, and fasting/weight reduced DIO mice. From this set, our bio

SUBMITTER: Raab RM 

PROVIDER: S-EPMC1201568 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature