A Novel Method for Preparing Uniform Micro-Sized Dry Powder Formulations, Including Aggregation-Controlled VHH.

ABSTRACT:

Background

The preparation of antibodies in powder form without changing their physicochemical properties may enable their use in new drug delivery system therapies or non-refrigerated storage. The variable domain of heavy-chain antibodies (VHHs) is more suited for this purpose than that of conventional antibodies because of VHHs' high thermal stability and ability to refold.

Methods

In this report, the fine droplet drying (FDD) process was selected as the powderization technique because of its favorable features, such as mild drying conditions and the generation of uniform particle sizes. The aggregation, binding, particle, and in vitro inhalation properties of the prepared VHH powders (VHHps) were evaluated.

Results

The amount of aggregated VHHs present in the VHHps

SUBMITTER: Moritani T 

PROVIDER: S-EPMC12015861 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature