Influence of LNA modifications on the activity of the 10-23 DNAzyme.

ABSTRACT: The 10-23 DNAzyme is a catalytic DNA molecule that efficiently cleaves RNA in the presence of divalent cations such as Mg²⁺ or Ca²⁺. Following their discovery, the 10-23 DNAzymes demonstrated numerous advantages that quickly led them to be considered powerful molecular tools for the development of gene-silencing tools. In this study, we evaluate the efficiency of the 10-23 DNAzyme and an LNA-modified analog in cleaving human MALAT1, an RNA overexpressed in cancer cells. First, we perform in vitro assays using a 20 nt RNA fragment from the MALAT1 sequence, with 2 mM and 10 mM Mg²⁺ and Ca²⁺ as cofactors, to evaluate how LNA modifications influence catalytic activity. We found that the activity is increased in the LNA-modified DNAzyme compare

SUBMITTER: Munoz-Gonzalez M 

PROVIDER: S-EPMC12016023 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature