Identification of Highly Repetitive Enhancers with Long-range Regulation Potential in Barley via STARR-seq.

ABSTRACT: Enhancers are DNA sequences that can strengthen transcription initiation. However, the global identification of plant enhancers is complicated due to uncertainty in the distance and orientation of enhancers, especially in species with large genomes. In this study, we performed self-transcribing active regulatory region sequencing (STARR-seq) for the first time to identify enhancers across the barley genome. A total of 7323 enhancers were successfully identified, and among 45 randomly selected enhancers, over 75% were effective as validated by a dual-luciferase reporter assay system in the lower epidermis of tobacco leaves. Interestingly, up to 53.5% of the barley enhancers were repetitive sequences, especially transposable elements (TEs), thus reinforcing the vital role of repetitive enhan

SUBMITTER: Zhou W 

PROVIDER: S-EPMC12016029 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature