From Glucose to Green Chemistry: Breakthrough in Microbial Production of Tartaric Semialdehyde.

ABSTRACT: L-(+)-tartaric acid (L-TA) is a crucial hydroxy carboxylic chelator with extensive applications in the food and pharmaceutical industries. The synthesis of L-TA from renewable biomass presents a promising approach to mitigating environmental impact and advancing green energy initiatives. Previous studies revealed that a mutant transketolase (TKTA_M) could catalyse the production of tartaric semialdehyde, a precursor to L-TA. This study focuses on the development of a Gluconobacter oxydans cell factory for tartaric semialdehyde production, employing a combination of metabolic engineering and a modular strategy. The genetically modified G. oxydans T strain exhibited robust expression of the tktA_M gene. The optimal pH and temperature for this strain were determined to be 6.0°C and 30°C, resp

SUBMITTER: Li S 

PROVIDER: S-EPMC12016104 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature