Circ-0069561 as a novel diagnostic biomarker for progression of diabetic kidney disease.

ABSTRACT:

Background

Circular RNAs (circRNAs) are non-coding RNAs that are key regulators of the initiation and progression of various human diseases. However, the role of circRNAs in diabetic kidney disease (DKD) remains unknown.

Methods

Whole high-throughput RNA sequencing (RNA-seq) was performed on kidney tissues from clinical DKD patients and controls. Circ-0069561 with significantly up-regulated expression level was selected by real-time PCR (RT-PCR) analysis. RT-PCR and fluorescent in situ hybridization (FISH) further validated the expression and subcellular localization of circ-0069561 in type 2 diabetic mice and DKD patients. The clinical significance of circ-0069561 in DKD was evaluated. The circRNA-miRNA-ferroptosis associated mRNA network was constructed. The biologi

SUBMITTER: Chen C 

PROVIDER: S-EPMC12016256 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature