Transcriptional regulation of phagocytosis-induced membrane biogenesis by sterol regulatory element binding proteins.

ABSTRACT: In the process of membrane biogenesis several dozen proteins must operate in precise concert to generate approximately 100 lipids at appropriate concentrations. To study the regulation of bilayer assembly in a cell cycle-independent manner, we have exploited the fact that phagocytes replenish membranes expended during particle engulfment in a rapid phase of lipid synthesis. In response to phagocytosis of latex beads, human embryonic kidney 293 cells synthesized cholesterol and phospholipids at amounts equivalent to the surface area of the internalized particles. Lipid synthesis was accompanied by increased transcription of several lipogenic proteins, including the low-density lipoprotein receptor, enzymes required for cholesterol synthesis (3-hydroxy-3-methylglutaryl CoA synthase, 3-hydrox

SUBMITTER: Castoreno AB 

PROVIDER: S-EPMC1201629 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature