Immobilization of alginate C-5 epimerases using <i>Bacillus subtilis</i> spore display.

ABSTRACT: Alginates are the most abundant polysaccharides found in brown seaweed, composed of (1→4)-linked β-D-mannuronate (M) and its C-5 epimer, α-L-guluronate (G). The G-blocks of alginate possess viscosifying and gelling properties, making alginates valuable industrial polysaccharides. Alginate epimerases are enzymes epimerizing M to G, enhancing the usability and value of alginate. The three alginate epimerases AlgE1, AlgE4, and AlgE6 were immobilized using Bacillus subtilis spores displaying the epimerases fused to the spore crust protein CotY. To our knowledge, this is the first display of immobilized alginate-modifying enzymes. Activity assays of the four AlgE4-displaying spore strains showed that AlgE4 produced MG-blocks from polyM alginate. AlgE4 was tested linked by its N- and C-te

SUBMITTER: Spannenkrebs JB 

PROVIDER: S-EPMC12016494 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature