Inoculum growth impacts <i>Salmonella</i> and Shiga-toxin producing <i>Escherichia coli</i> resilience on wheat grain.

ABSTRACT: Previous studies have shown that using a surface growth method for preparing inoculum impacted pathogen population stability and inactivation kinetics. Here, we quantified the desiccation survival and responses to tempering treatment of Salmonella and Shiga-toxin producing Escherichia coli (STEC) on wheat grain when grown using five different methods: broth, lawn-aerobic, lawn-anaerobic, lawn-acid-adapted, and lawn-low inoculum. Three strains of Salmonella and STEC each were individually inoculated onto wheat grain, conditioned to 0.45 a_w and stored up to 24 weeks. Pathogen survival curves on grain were different when inoculated with a lawn-grown culture compared to broth-grown. Acid adaptation of STEC led to increased (P < 0.001) tolerance to

SUBMITTER: Lin Y 

PROVIDER: S-EPMC12016515 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature