Polyyne production is regulated by the transcriptional regulators PgnC and GacA in <i>Pseudomonas protegens</i> Pf-5.

ABSTRACT: Polyynes produced by bacteria have promising applications in agriculture and medicine due to their potent antimicrobial activities. Polyyne biosynthetic genes have been identified in Pseudomonas and Burkholderia. However, the molecular mechanisms underlying the regulation of polyyne biosynthesis remain largely unknown. In this study, we used a soil bacterium Pseudomonas protegens Pf-5, which was recently reported to produce polyyne called protegenin, as a model to investigate the regulation of bacterial polyyne production. Our results show that Pf-5 controls polyyne production at both the pathway-specific level and a higher global level. Mutation of pgnC, a transcriptional regulatory gene located in the polyyne biosynthetic gene cluster, abolished polyyne produc

SUBMITTER: Mwanza C 

PROVIDER: S-EPMC12016544 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature