A suite of ddPCR assays targeting microbial pathogens for improved management of shellfish aquaculture.

ABSTRACT: The shellfish aquaculture industry is one of the fastest-growing sectors of global food production, but it is currently facing major challenges stemming from microbial pathogens. This study presents an optimized and validated suite of droplet digital PCR (ddPCR) assays using water samples proximal to oyster farms in North Carolina to quantify pathogens relevant to the aquaculture industry. Two of the molecular assays enable the quantification of the pathogens, Vibrio parahaemolyticus and Perkinsus marinus, that threaten human health and oyster performance, respectively. This work also introduces two ddPCR assays that enable the simultaneous quantification of at least nine ecologically relevant Vibrio spp. using only two sets of primers and probes targeting the glycosyl

SUBMITTER: Ciesielski M 

PROVIDER: S-EPMC12016556 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature