Inter3D: Capture of TAD Reorganization Endows Variant Patterns of Gene Transcription.

ABSTRACT: Topologically associating domain (TAD) reorganization commonly occurs in the cell nucleus and contributes to gene activation and inhibition through the separation or fusion of adjacent TADs. However, functional genes impacted by TAD alteration and the underlying mechanism of TAD reorganization regulating gene transcription remain to be fully elucidated. Here, we first developed a novel approach termed Inter3D to specifically identify genes regulated by TAD reorganization. Our study revealed that the segregation of TADs led to the disruption of intrachromosomal looping at the myosin light chain 12B (MYL12B) locus, via the meticulous reorganization of TADs mediating epigenomic landscapes within tumor cells, thereby exhibiting a significant correlation with the down-regulation of its transcri

SUBMITTER: Ding T 

PROVIDER: S-EPMC12016567 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature