<i>In Silico</i> Driven Multi-Epitope Subunit Candidate Vaccine against Bovine Tuberculosis.

ABSTRACT: Bovine tuberculosis (bTB), caused by Mycobacterium bovis, poses significant zoonotic and economic challenges globally. The current prevention and treatment options are limited and increasingly complicated by the emergence of multidrug-resistant strains. This study employs reverse vaccinology and immunoinformatics to design a multi-epitope subunit vaccine targeting the MPB83, ArfA, DnaK, GrpE, and LpqH proteins of M. bovis. The T-cell and B-cell epitopes of the candidate vaccine were predicted and evaluated for antigenicity, allergenicity, and toxicity. The promising epitopes were then assembled into three vaccine constructs (bTBV1, bTBV2, and bTBV3) using appropriate adjuvants, pan HLA DR-binding epitope (PADRE), and linkers. The constructs were analyzed for physicochemical p

SUBMITTER: Faysal MA 

PROVIDER: S-EPMC12016833 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature