Real-Time Reverse Transcription Multienzyme Isothermal Rapid Amplification for Rapid Detection of African Horse Sickness Virus.

ABSTRACT: African horse sickness (AHS) is an acute infectious disease of equids caused by the AHS virus (AHSV), which can cause up to 90% mortality in naive horses. Reliable and rapid diagnosis is crucial for the surveillance and control of AHSV. As one of the AHSV detection methods recommended by World Organization for Animal Health (WOAH), the RT-qPCR assay has the drawbacks such as complex operation, expensive instruments, and long detecting time, which limit its application in simple laboratories or outdoors. In this study, a real-time reverse transcription multienzyme isothermal rapid amplification (RT-MIRA) assay was established to detect AHSV. Primers and exo-probes were designed, synthesized, and screened based on the conserved regions of the AHSV Seg-7 gene. A series of experiments w

SUBMITTER: Huang C 

PROVIDER: S-EPMC12016855 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature